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Safe and efficient novel methodologies for labelling of N- and O-glycans for chromatographic, electrophoretic and spectroscopic analysis (10 NL 60AF 3GNG)
Protein-linked glycans regulate many biological processes. O-glycosylation is a.o. important in mucosal secretion, N-glycosylation in the folding of proteins. Conventional labelling of N-glycans produces the toxic hydrogen cyanide gas, while labelling of O-glycans is slow and requires expensive equipment. A Dutch Medical center has developed, a non toxic labelling process for N-glycans and a faster simpler labelling method for O-glycans. A commercial partner is sought.
Country: Netherlands
Type: OFFER
Date: 17.08.2011
Scientists from a Dutch Medical Center have developed novel methodologies for the labeling of glycans.
Protein-linked glycans are involved in important biological processes such as cellular differentiation, recognition and adhesion, receptor activation, and molecular trafficking. Moreover, O-glycosylation specifically plays an important role in mucosal secretion, whilst N-glycosylation is important for the folding of eukaryotic proteins.
Research on the role of glycans in drug therapy and their use as biomarkers requires fast and sensitive high-throughput glycoanalytical methods.
The most common approaches include HPLC and capillary electrophoresis both of
which are mostly coupled to UV, fluorescence or mass spectrometric detection. In most approaches, glycans are labeled prior to analysis.
Customary methodologies for labeling O-glycans are demanding in terms of both time and equipment. Conventionally N-glycans have been labeled by reductive amination using sodium cyanoborohydride (NaBH3CN) as reducing agent. This
reaction releases the toxic, volatile compound hydrogen cyanide as a
side product.
Scientists from a Dutch University Medical Center have now developed a non-toxic approach for the labeling of N-glycans. For the release and labeling of O-glycans a much faster method has been developed, which can be followed by a simple sample work-up and mass spectrometric analysis.
Innovative Aspects:
- scientists have now developed a non-toxic approach for the labeling of N-glycans.
- for the release and labeling of O-glycans a much faster method has been developed, which can be followed by a simple sample work-up and mass spectrometric analysis.
Advantages:
- Labeling of N-glycans: non toxic end product.
- Labeling of O-glycans: faster and easier methodology.
Degree of development:
Patents/Rights: Patent(s) applied for but not yet granted
Requested Cooperation: License Agreement, Technical consultancy, Quality control, Joint further development, Testing of new applications, Adaptation to specific needs
- Type of partner sought: suppliers of laboratory materials and kits in the
field of analytical chemistry, glycoproteomics,
HPLC and MAS.
- Specific area of activity of the partner: analytical chemistry, glycoproteomics,
HPLC and MAS.
- Task to be performed by the partner sought: developing the procedure into a marketable product and at a later stage marketing the product.
Type of Organisation:
Status: NEW
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